CD45 is a family of single chain transmembrane glycoproteins, consisting of at least four isoforms which share a common large intracellular domain. Their extracellular domains are rod shaped, heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1q31-32.
CD45 is exclusively expressed in haematolymphoid cells. Almost all haematolymphoid cells, including precursor cells and mature B- and T-lymphocytes, granulocytes, monocytes/histiocytes and interdigitating reticulum cells and follicular dendritic cells express CD45. This protein is detected in the large majority of haematolymphoid neoplasms, i.e., leukemias and malignant lymphomas. Overall, about 90% of malignant lymphomas are CD45 positive.
CD45 is an important marker in the primary tumor screening panel in order to identify haematolymphoid differentiation. Loss of CD45 in precursor B-cell neoplasms is a negative prognostic parameter.
Specifications
Catalog No.
BX50068
Clone No.
BP6073
Application
IHC-P
Subcellular location
Membrane
Control
Tonsil
Recommended method
HIER
Volume
100μl/vial, 1ml/vial
Dilution
1:100-1:200
Immunogen
Recombinant fragment within Human CD45 aa 1 to aa600.
Reference
1.Dahlke MH et al. Leuk Lymphoma. 2004 Feb;45(2):229-36.
2.Gaertner EM et al. Am J Clin Pathol. 2001 Apr;115(4):589-97.