Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits. Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament. Neurofilament antibody mainly stains neurons in tissue sections of brain and other tissues. Neurofilament has been shown to react with neuroblastomas, gangliomas, pheochromocytomas, Merkel cell tumors and carcinoid tumors.
Specifications
Catalog No.
BX50249
Clone No.
BPM6226
Application
IHC-P
Subcellular location
Cytoplasm
Control
Brain
Recommended method
HIER
Volume
100μl/vial, 1ml/vial
Dilution
1:100-1:200
Immunogen
Recombinant fragment within Human Neurofilament was used as an immunogen.
Reference
1. Franquemont DW, et al. Am J Clin Pathol 1994 Aug;102(2):163-70.
2. Diepholder HM, et al. Cancer 1991 Nov 15;68(10):2192-201.