The topoisomerase II-α isoform is a 170 kDa nuclear protein and plays an important role in DNA synthesis and RNA transcription, as well as chromosomal segregation during mitosis.Topoisomerase II-α is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells, and has been shown to be over-expressed in many human cancers.Decreased expression of Topoisomerase II-α is the predominant mechanism of resistance to several chemotherapeutic agents.
Synthetic peptide corresponding to Topo II α residues within aa1431-1531 of Topo II α was used as an immunogen.
1.Lynch BJ, et al, Hum Pathol. 1997 Oct;28(10):1180-8
2.Gupta D, et al, Appl Immunohistochem Mol Morphol. 2001 Sep;9(3):215-21.